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Lamp2 western blot

WebbSummary. LAMP2 antibody detects lysosome-associated membrane protein 2 (LAMP-2, or CD107b), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP-2 has a similar distribution to LAMP-1 in endolysosomal and autophagic organelles. Moreover, like LAMP-1, LAMP-2 has been used to study … WebbGalectin Therapeutics hpde cells Hpde Cells, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.

Identification of lysosome‐targeting drugs with anti‐inflammatory ...

WebbObjective: Autophagy is a highly conserved degradation pathway in cells, which has been involved in many physiological processes and implicated in human age-related diseases. However, autophagy activities have not been systemically investigated with human tissues and cells. Methods: Lysosomal associated membrane protein-2 (LAMP-2) protein is … WebbWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody … charles belden the rescue https://i2inspire.org

Frontiers Nascent Glycoproteome Reveals That N-Linked …

WebbDetection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAMP2/CD107b Monoclonal Antibody (Catalog # MAB6228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # … WebbIn age-related macular degeneration (AMD), hydroquinone (HQ)-induced oxidative damage in retinal pigment epithelium (RPE) is believed to be an early event contributing to dysregulation of inflammatory cytokines and vascular endothelial growth factor (VEGF) homeostasis. However, the roles of antioxidant mechanisms, such as autophagy and … WebbAnti-LAMP2B antibody - Lysosome Marker Applications: Western Blot (WB) Reactivity: Human (Hu) Conjugate/Tag: Unconjugated Quantity: 100 UG Citations: (26) Supplier … charles bell attorney

Lamp2 Antibody (GL2A7) - DSHB

Category:Anti-LAMP2 antibody (GTX103214) GeneTex

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Lamp2 western blot

Molecular characterization of sub-RPE deposits in Lamp2 KO mice.

WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and … WebbFigure 1 The expression of PHLPP in bortezomib-resistant MM cells. (A) Western blot analyses of the expression of PHLPP, LAMP2, and key autophagy signaling molecules in bone marrow samples from patients with MM (upper), and quantification of the bands (lower).(B) Western blot analyses of the expression of PHLPP, LAMP2, and key …

Lamp2 western blot

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WebbWe next characterized molecules in sub-RPE deposits in Lamp2 KO mice by Western blotting. Key proteins found in human drusen, such as apolipoprotein E (APOE), clusterin (CLU), and vitronectin (VTN ... WebbDetection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 …

WebbWestern blotting showed that the expression of glycosylated LAMP2 in Huh7 cells was considerably reduced under NGI-1 treatment in a concentration-dependent manner (Supplementary Figure S1). To further confirm the suppression extent of LAMP2 by NGI-1, fine mapping of the N-glycosylation profile of LAMP2 was achieved based on lysosome … WebbExosome quality was assessed by Coomassie staining and Western blotting for CD9, LAMP2 exosomal markers and a negative marker Grp94. Results: Correlation between …

WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. WebbLAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) Positive control WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. IHC-P: Human breast adenocarcinoma tissue. General notes The Life Science industry has been in the grips of a reproducibility crisis for a …

WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and …

WebbDetection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, JEG-3 human epithelial choriocarcinoma cell … charles bell attorney savannah gaWebbFabgennix Anti-LAMP2 Polyclonal, Catalog # LAMP2-BIOTIN. Tested in Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (ELISA) and Dot blot (DB) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 200 µL purified antibody (0.5-1.5 mg/mL). charles belle belfortWebb27 juli 2016 · Mireskandari et al. (1996) demonstrated a specific interaction between Tax1 and TRX by coimmunoprecipitation and Far Western blot analyses. Using immunohistochemical analysis, Sbrissa et al. (2004) showed that human VAC14 colocalized with mouse Pikfyve in the perinuclear region following cotransfection of … charles belle gary police officerWebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate … harry potter chess game movesWebbDetection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 … charles bell exeter nhWebbFor each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 … harry potter cherry wood wandWebb27 aug. 2024 · e Co-immunoprecipitation of Gal-9 and Lamp2 or M6PR in CMT93 cells, analyzed by Western blot analysis. f Lysosomal hydrolase activity of the indicated … harry potter chess board