Thioglycolate-elicited peritoneal macrophages
WebMacrophages constitute a very versatile population of phagocytic cells present in most tissues, albeit in different forms such as microglia, osteoclasts, and Kupffer cells. ... WebIn contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7 −/-macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3–PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception ...
Thioglycolate-elicited peritoneal macrophages
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WebDec 20, 2011 · Thioglycolate-elicited peritoneal macrophages prepared after four weeks of feeding, and then challenged with LPS (10 ng or 100 ng) resulted in increases of 55% and 73%, respectively in the production of NO of 46-week-old compared to 8-week-old mice fed control diet alone (respective control groups), without affecting the secretion of TNF-α ... WebJan 13, 2003 · Thioglycolate-elicited peritoneal macrophages were cultured in DMEM/10% LPDS and treated with vehicle or 2 μM GW3965 for 18 h before stimulation with 100 ng/ml LPS for 6 h. Total RNA was reverse ...
WebTreatment of thioglycollate-elicited, C57Bl/6 murine peritoneal macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) consistently results in altered 32Pi … Webfor LC3–PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7−/- macrophages exhibited no LC3-II, even under …
WebApr 14, 2009 · Surprisingly, although it has been reported that thioglycolate-elicited peritoneal macrophages do not express PD-1 , we observed a significant up-regulation of PD-1 expression on peritoneal macrophages at 12, 24, and 48 hours post-CLP (Fig. 2 A and B). In contrast, the augmentation of PD-1 expression on splenic T cells and B cells during … WebMacrophages were separated from thioglycollate-elicited peritoneal cells. Lymphocytes were purified from peripheral blood and were then stimulated w … Using tumor necrosis factor (TNF)-sensitive L929 cells, TNF-like activity was assayed in the supernatants of feline macrophage and lymphocyte cultures.
WebJan 1, 1998 · Similarly, thioglycolate-elicited mouse peritoneal macrophages were cultured for 18 h in the presence or absence of 2 µM 15d-PGJ 2. Total RNA was isolated and subjected to analysis by RNase ...
WebFeb 17, 2024 · Thioglycolate-Elicited Peritoneal Macrophages Isolation. Thioglycolate (TG) (3%) was intraperitoneally injected into mice which were sacrificed by cervical dislocation 4 days later. The TG-elicited peritoneal macrophages (TGPMs) were isolated by washing buffer (sterile PBS plus 5% newborn calf serum and 0.5 mM EDTA). ... is chalk limestoneWebJan 1, 2013 · There are four commonly utilized macrophage preparations: primary resident macrophages, thioglycollate-elicited macrophages, bone marrow-derived macrophages, … is chalk man made or naturalWebApr 14, 2009 · Surprisingly, although it has been reported that thioglycolate-elicited peritoneal macrophages do not express PD-1 , we observed a significant up-regulation of … is chalk marineWebThe M3/38 monoclonal antibody specifically recognizes Galectin-3 (Gal-3 or gal3) which is also known as Galactose-specific lectin 3, Mac-2, MAC2, and Carbohydrate-binding protein 35 (CBP 35). Galectin-3 is an ~30-35 kDa protein that includes an N-terminal proline-rich tandem repeat domain as well as a C-terminal region with one carbohydrate recognition … ruth moxeyWebAug 1, 2012 · Thioglycollate-elicited peritoneal cells are a common source of macrophages for various in vitro assays, including stimulation with TLR ligands, cell signaling assays, phagocytosis, toxicology studies, and cytokine/chemokine production. The most common method for enrichment of cultured thioglycollate-elicited peritoneal cells is adherence. is chalk an element compound or mixtureWebApr 9, 2024 · Moreover, peritoneal macrophages in the fibrotic peritoneum are considered the major source of inflammatory and pro-fibrotic cytokines, such as transforming growth … ruth moversruth moxon