Thioglycollate-elicited macrophages

Author: zstp

August undefined, 2024

WebAbstract. Brewer thioglycolate-elicited mouse peritoneal macrophages were as active as resident peritoneal macrophages in the phagocytosis of opsonized Staphylococcus epidermidis but were unable to kill ingested microorganisms. This decreased functional activity was restricted to Brewer thioglycolate-elicited macrophages, since peritoneal ... WebFeb 28, 1997 · Abstract. Thioglycollate medium (TGM) is widely used as a stimulatory agent to induce non-infectious peritoneal inflammation for elicitation of macrophages from …

ATG7 is dispensable for LC3–PE conjugation in thioglycolate …

WebResident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or … shown mixed results

Effect of glycolysis inhibition in macrophage phagocytosis and ...

WebSep 11, 2014 · H. In a separate experiment, CD11b + F4/80 + macrophages were sorted from the same naïve or thioglycollate-elicited peritoneal cells. Lyz2 gene expression was found to be lower in thioglycollate-elicited macrophages than naïve macrophages (n = 2–5 for 2 independent experiments). Data shown are mean ±SEM and represent two independent ... WebMar 7, 2011 · Neutrophils and monocytes express more S100A8 and S100A9 than do macrophages. Thioglycollate-elicited neutrophils (Neut) and macrophages (Mac) were obtained from C57BL/6 mice. S100A8 (A; n=6) and S100A9 (B; n=6) mRNA levels were measured by real-time polymerase chain reaction. Cellular S100A9 protein and β-actin … WebResident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. shown morin

Detection of tumor necrosis factor activity in the supernatants of ...

BALB/c Mouse Peritoneal Macrophages - Thioglycollate-elicited …

WebMay 9, 2002 · a, Thioglycollate-elicited peritoneal macrophages or P388D1 cells were surface-labelled with biotin, immunoprecipitated with the 2422 antibody, and analysed by western blotting with peroxidase ... WebWhen a macrophage-like cell line (J774), lacking in MFR, was purposely selected to test the previous findings, as expected, the attachment of avirulen t promastigotes (UR6) onto the cell line was found to be negligible when compared to the peritoneal macrophages. Thus, it appears that avirulent leishmania promastigotes probably shown lorenzo cooperWebJan 1, 2013 · There are four commonly utilized macrophage preparations: primary resident macrophages, thioglycollate-elicited macrophages, bone marrow-derived macrophages, … shown mercy

"WebSep 20, 2015 · Here we described a method for the isolation of peritoneal macrophages from thioglycollate-elicited mice and alternative activation by stimulation with IL-4. " - Thioglycollate-elicited macrophages

Thioglycollate-elicited macrophages

Deoxycytidine excretion by mouse peritoneal macrophages: its

WebI have been trying to isolate protein from thioglycolate-elicited peritoneal macrophages. After harvest, I plate cells at ~1-2mil/well, wash out non-macrophages the next day, and wait till ... WebAug 23, 2024 · Use of Thioglycollate-elicited macrophages (TGEMs) has been has been explicitly highlighted throughout the manuscript, and we have included this detail in the …

Did you know?

WebTreatment of thioglycollate-elicited, C57Bl/6 murine peritoneal macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) consistently results in altered … WebApr 16, 2024 · Fra-1 mRNA levels in thioglycollate-elicited macrophages peaked prominently at 1 hour, and protein levels increased 2 hours after AC or LPS challenge in vitro (Figure 2, A and B). Fra-1 expression in BMDMs following LPS stimulation also peaked 1 hour after challenge in a manner similar to that seen in thioglycollate-elicited …

WebMacrophages (Mφ) are central players in both the innate and adaptive immune systems and are attractive cells to study in culture because of their wide range of cellular functions. … WebIn contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7−/- macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3–PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception ...

WebFeb 10, 2024 · The cells can be collected under non-elicited (resident) or elicited (e.g., with Brewer thioglycollate broth injection) conditions, and their phenotype and functions differ. … WebAug 20, 2024 · The EGF-like domain repeats competitively impair apoptotic cell uptake by thioglycollate-elicited peritoneal macrophages in an in vivo animal model. At least four tandem repeats of the EGF-like domain containing an atypical EGF-like domain are required for binding to phosphatidylserine [ 50 ].

WebNov 11, 2024 · Thioglycollate-elicited macrophages were allowed to attach on a coverglass for 30 min and then fixed by 4% PFA in PBS for 1 h. Cells were stained for 30 min with 0.3% ORO solution in 60% isopropanol. The mean area and integrated optical density of ORO-stained region per cell were quantified with 200 representative cells using ImageJ …

WebProduct Description. C57BL/6 Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at … shown notes ictWebDeoxycytidine was found in the culture medium of thioglycollate-elicited mouse peritoneal macrophages, along with thymidine, which was shown by others to be a possible immunoregulatory substance. The identification of deoxycytidine was based on: (1) cochromatography with the authentic compound in four different solvents, (2) UV … shown mendes holWebthioglycollate-elicited macrophages expressed Ki67 and only 0.2% were in S phase (Fig. 2A). Notably, pleural macrophages isolated 10 days after infection with L. sigmodontis exhibited a substantial increase in the frequency of dividing cells. On average, 38% of cells in the pleural cavity were positive for Ki67 and 6% were in S phase (Fig. 2B). shown more synonymWebMay 6, 2024 · After treatment with thioglycollate and IL-4c, Bhlhe40 – / – mice had severely reduced proportions of BrdU + and pHH3 + thioglycollate-elicited macrophages … shown nameWebFeb 10, 2024 · Elicited macrophages were harvested from mice which had received a 1.5 ml intraperitoneal administration of 3% Brewer thioglycollate broth (Sigma, UK) 72 h prior … shown off意味WebAbstract. Brewer thioglycolate-elicited mouse peritoneal macrophages were as active as resident peritoneal macrophages in the phagocytosis of opsonized Staphylococcus … shown my pc.comWebBALB/c Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at passage 0 are cryo … shown mades vedio